pax7 s 10 14 21 mouse anti myhc embryonic  (Developmental Studies Hybridoma Bank)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Extraocular Muscle Reveals Selective Vulnerability of Type IIB Fibers to Respiratory Chain Defects Induced by Mitochondrial DNA Alterations

doi: 10.1167/iovs.61.12.14

Figure Lengend Snippet: Type IIB fibers show selective vulnerability to develop COX deficiency . ( A ) Representative pictures from consecutive sections stained for sequential COX / SDH histochemistry and MyHC I, IIA, and IIB (triple-immunofluorescence) of 12 months and ( B ) 24 months old K320E-Twinkle skm . Numbers highlight the COX − fibers and the corresponding fiber type on the consecutive section. ( C ) Representative pictures for sequential COX-SDH histochemistry and myosin heavy chain embryonic and ( D ) for extraocular muscle specific myosin heavy chain. Numbers label same fibers in the corresponding images. ( E ) Proportions of different fiber types that were COX deficient in selected rectus muscles of K320E-Twinkle skm at the age of 12 and 24 months. Scale bar, 100 µm. Data expressed as mean ± SEM, N = 4 to 5 animals per group. Two-way ANOVA., Interaction: ** P < 0.01; Fiber Type: **** P < 0.0001; Age: P = 0.0616.

Article Snippet: The sources and dilutions of antibodies against MyHC isoforms were as follows: mouse anti-embryonic MyHC (1:20; embryonic-MyHC, IgG; F1.652), mouse anti-EOM-specific MyHC (1:30; Eom-specific-MyHC, IgM; 4A6), mouse anti-type I MyHC (1:50; I-MyHC, IgG2b; BA-D5), mouse anti-type IIa MyHC (1:100; IIa-MyHC, IgG; SC-71), and mouse anti-type IIb MyHC (1:20; IIb-MyHC, IgM; BF-F3); these were obtained from the Developmental Studies Hybridoma Bank (DSHB, University of Iowa, Iowa City, IA, USA).

Techniques: Staining, Immunofluorescence, Muscles

Journal: Investigative Ophthalmology & Visual Science

Article Title: Extraocular Muscle Reveals Selective Vulnerability of Type IIB Fibers to Respiratory Chain Defects Induced by Mitochondrial DNA Alterations

doi: 10.1167/iovs.61.12.14

Figure Lengend Snippet: Respiratory chain defects alter fiber type composition of extraocular muscles and retractor bulbi . ( A ) Representative images of whole extraocular muscle sections stained for MyHC I, IIA, and IIB (triple-immunofluorescence) at 12 and 24 months. ( B ) Proportions of type I, IIA, and IIB in global and orbital layer of recti muscles and retractor bulbi. ( C ) Proportions of EOM-specific and embryonic fiber types in global and orbital layer of recti muscles and retractor bulbi. Data expressed as mean SEM, N = 4 to 5 animals per group. Two-way ANOVA, Tukey multiple comparison test. ** P < 0.001, *** P < 0.001. Scale bar, 200 µm.

Article Snippet: The sources and dilutions of antibodies against MyHC isoforms were as follows: mouse anti-embryonic MyHC (1:20; embryonic-MyHC, IgG; F1.652), mouse anti-EOM-specific MyHC (1:30; Eom-specific-MyHC, IgM; 4A6), mouse anti-type I MyHC (1:50; I-MyHC, IgG2b; BA-D5), mouse anti-type IIa MyHC (1:100; IIa-MyHC, IgG; SC-71), and mouse anti-type IIb MyHC (1:20; IIb-MyHC, IgM; BF-F3); these were obtained from the Developmental Studies Hybridoma Bank (DSHB, University of Iowa, Iowa City, IA, USA).

Techniques: Muscles, Staining, Immunofluorescence, Comparison